Tag
Reverse Vaccinology
Bioinformatics How to Predict Conformational B-Cell Epitopes Run ElliPro, DiscoTope 3.0 and SEPPA 3.0 on a structure, read their scores correctly, and avoid the residue-numbering trap. Ragini Mishra 17 min read Bioinformatics How to Predict IFN-gamma, IL-4 and IL-10 Inducing Epitopes Screen your shortlisted MHC class II binders for IFN-gamma, IL-4 and IL-10 induction, and learn when a high score is a reason… Ragini Mishra 15 min read Bioinformatics How to Select Target Antigens for Reverse Vaccinology Start a vaccine design project right. Five filters, in order, that cut a whole proteome down to the proteins worth predicting epitopes… Ragini Mishra 11 min read Bioinformatics How to Check Epitope Allergenicity, Toxicity and Antigenicity: VaxiJen, AllerTOP and ToxinPred Screen your epitope shortlist properly: which server to run first, which threshold to set, and what to do with a borderline score.… Ragini Mishra 14 min read Bioinformatics How to Design a Multi-Epitope Vaccine Construct: Linkers, Adjuvant and Order Join your shortlisted epitopes into one designed protein: which linker goes where, why order matters, and how to validate the sequence. Build… Ragini Mishra 14 min read Bioinformatics How to Predict T-Cell Epitopes and MHC Binding with NetMHCpan and IEDB Pick an HLA panel, set the right peptide length, and filter on percentile rank. Turn thousands of NetMHCpan rows into a defensible… Ragini Mishra 17 min read Bioinformatics How to Predict B-Cell Epitopes with BepiPred and ABCpred: A Step-by-Step Guide Run BepiPred and ABCpred on the same antigen, read per-residue scores against the threshold, and keep only consensus peptides. Start with this… Ragini Mishra 15 min read