Tag
Vaccine Design
Bioinformatics How to Select Target Antigens for Reverse Vaccinology Start a vaccine design project right. Five filters, in order, that cut a whole proteome down to the proteins worth predicting epitopes… Ragini Mishra 11 min read Bioinformatics ProtParam for a Vaccine Construct: Reading Every Number Run ProtParam on your multi-epitope construct and learn what each number means, which cutoff is real, and what to change when a… Ragini Mishra 16 min read Bioinformatics How to Run an IEDB Population Coverage Analysis for Your Epitope Set Run the IEDB Population Coverage tool step by step, format the input correctly, read PC90 properly, and fix thin coverage in South… Ragini Mishra 13 min read Bioinformatics How to Codon Optimize a Vaccine Construct for E. coli Expression: Codon Usage, CAI and Restriction Sites Learn how to codon optimize a multi-epitope vaccine construct for E. coli: reverse translation, CAI, GC3 and the restriction-site check before you… Ragini Mishra 15 min read Molecular Modeling How to Run an MD Simulation of a Vaccine Construct-TLR Complex in GROMACS: Setup, Length and What to Report Take a docked vaccine construct into GROMACS: which pose to carry forward, force field and box setup, how many replicas to run,… Ragini Mishra 17 min read Bioinformatics How to Dock a Multi-Epitope Vaccine Construct with TLR4: ClusPro, HDOCK and HADDOCK Compared Dock a screened vaccine construct against TLR4 the way a reviewer reads it: pick the PDB entry, prep the files, compare three… Ragini Mishra 16 min read Bioinformatics How to Check Epitope Allergenicity, Toxicity and Antigenicity: VaxiJen, AllerTOP and ToxinPred Screen your epitope shortlist properly: which server to run first, which threshold to set, and what to do with a borderline score.… Ragini Mishra 14 min read Bioinformatics How to Design a Multi-Epitope Vaccine Construct: Linkers, Adjuvant and Order Join your shortlisted epitopes into one designed protein: which linker goes where, why order matters, and how to validate the sequence. Build… Ragini Mishra 14 min read Bioinformatics Immunoinformatics: What It Is and How It’s Helping Design Smarter Vaccines Vaccines are among the greatest achievements in medical science, saving millions of lives every year. But did you know the way we… Dr. Abdul Rajjak Shaikh 3 min read Updated Publications Immunoinformatics-Aided Design and In Vivo Validation of a Peptide-Based Multiepitope Vaccine Targeting Canine Circovirus Abstract Canine circovirus (CanineCV) is a deadly pathogen affecting both domestic and wild carnivores, including dogs. No vaccine against CanineCV is available… Stemskills Lab 1 min read Updated