Immunoinformatics Aided Design and In-Vivo Validation of a Cross-Reactive Peptide Based Multi-Epitope Vaccine Targeting Multiple Serotypes of Dengue Virus

Dengue affects over 400 million people a year, and current vaccines remain limited. This study uses immunoinformatics to design a multi-epitope vaccine covering all four dengue serotypes, selecting ten conserved, interferon-inducing epitopes. Molecular docking and molecular dynamics predicted stable binding to the TLR5 receptor, and, unusually, one epitope was synthesised and shown to raise antibodies in a rabbit model, supporting the design’s immunogenicity.